Magda Gladysz – Novel Blood-Brain-Barrier in vitro models

I am working on Melt electrowriting (MEW) of scaffolds for recapitulation of the blood-brain barrier (BBB) in dynamic on-chip cancer models.

This project aims to design a thin polymeric membrane on which relevant for BBB cells form confluent layers with tight junctions. The membrane will serve as a minimalistic supporting scaffold, favouring cell-cell interactions instead of cell-material interactions. The properties of the membranes, such as architecture, porosity and fiber thickness, are investigated in relation to cell behaviour and tissue formation. For the in vitro simulation to be completed, dynamic conditions need to be added. The innovative microfluidics approach offers a way to imitate organ environment in a highly accurate manner by incorporating different stimuli in the cell microenvironment. Thus, in combination with MEW, it provides an opportunity to build an accurate cancer-on-a-chip model.